Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. The plate is set up for reading and images are acquired according to specified parameters. Transcreener® ADP2 TR-FRET assay. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Lysis buffer 1X for assay protocol on tissues, two-plate assay protocol on adherent cells, and standard dilution. TR-FRET was measured on the SpectraMax i3x and iD5 readers (see Table 1 for instrument settings). Due to the chemical and photophysical. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. → Incubate 2h (if the detection is not sensitive enough, it is better to incubate 20h). It is used as a positive control for Gs applications ( biological models validation,Detection of Total CDK12 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Download the brochure to learn how this compact and robust device can handle various detection modes, plate formats, and software options. HTRF® CertifiedCertified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. The PHERAstar ® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. FOR HTRF CERTIFIED READER . This is thanks to a dedicated Alpha Technology laser which outperforms any xenon-based light source by exciting donors at 680 nm. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. 96-well culture plateThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. FOR HTRF CERTIFIED READER. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Plate 25 μL of cells in. FOR HTRF CERTIFIED READER. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:320/40 HTRF Eu cryptate 665/7uv Wide Filter 0. For fluorescent assays, black plates absorb light and reduce background and crosstalk. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. The Negative control is used to check the non-specific signal. For more information about HTRF. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. Assay setup for a 384-well low-volume plate. FOR HTRF CERTIFIED READER. The LVis Plate contains 16 spots which can be loaded with 2 microliters of sample for subsequent absorbance measurements. It accepts all types of microtitration plate. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. For more. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersFOR HTRF CERTIFIED READER. According to the manufacturers protocol, this protein assay is linear in the range of 0. If not, our scientific team will help you choose the instrument that best suits your needs and budget. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Make sure the setup for Tb Cryptate is used. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same . It can also be further streamlined to a one-. February 23, 2010, WINOOSKI VT, USA - The Synergy TM H4 Hybrid Microplate Reader from BioTek Instruments is now HTRF® certified. Live Viewer for cell-based assays. Read other about this measurement approach here. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). FOR HTRF CERTIFIED READER. ** The Detection Buffer is used to prepare working solutions of acceptor and donor reagents. For a list of HTRF-compatible readers and set-up recommendations, please visit . For more information about microplate recommendations, please visit our website • Store the kit at -16°C. Simply place the module into one of the five module bays and read the assay. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. High Speed Monochromator offers a typical wavelength accuracy of <0. 96-well culture plateHowever, time-resolved fluorescence (TRF & TR-FRET) assays are used in conjunction with fluorophores that have a longer half-life. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non-specific short-lived emissions. PROTOCOL AT A GLANCE Do not pre-mix the d2 and Cryptate solutions prior to dispensing. Dual monochromators for assay flexibility With. As shown in Fig. . Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. -Certified Reader** *Assay volumes can be adjusted proportionally to run the assay in 96- or 1536-well microplates. After defining the objectives and their locations in Gen5: 1. At that stage, the analyte is sandwiched between the 2 antibodies. Because of its homogeneous and fast nature, the assay is a preferred method to determine the protein concentration of samples. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. + 4 µL HTRF pre-mixed antibodies. This protocol gives the cells viability and confluence to be monitored. . FOR HTRF CERTIFIED READER. Conclusion. Homogeneous Time-Resolved Fluorescence (HTRF) . • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readers HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. e. FOR HTRF CERTIFIED READER For. But sometimes these parameters are not sufficient enough. 96-well culture plate 384SV detection plate- Add . FOR HTRF CERTIFIED READER. Application Area: absorbance, fluorescence, luminescence, and homogeneous time resolved fluorescence assay development. Preconfigured HTRF protocols in SoftMax ® Pro Software 7. FOR HTRF CERTIFIED READER For more information about HTRF. Sensitive, accurate and reliable detection is critical across cell, protein and genomic analysis applications in drug discovery and development. Quantify up to 10 analytes per well. Make sure the setup for Eu3+ Cryptate is used. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. Bench top solutions for cell based assays. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. The HTRF ratio obtained represents the total binding. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. ratio, high sensitivity, wide dynamic range, superior stability, and excellent flexibility. Eu cryptate emission level). Read & analyse. + 4 µL HTRF ® pre-mixed antibodies . These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:FOR HTRF CERTIFIED READER. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersONE-PLATE ASSAY PROTOCOL: Small volume detection plate. The plate is set up for reading and images are acquired according to specified parameters. For a list of HTRF-compatible readers and set-up recommendations, please visit . As shown in Fig. These plates can be read on all HTRF certified readers, thus making their implementation in. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. For the standard curve described, all wells of the microplate are read. FOR HTRF CERTIFIED READER. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. 96-well culture plateFOR HTRF CERTIFIED READER. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Read & analyse. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. For more information about HTRF. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. HTRF 96-well low volume plate *To prepare working standard solutions, culture medium can be an alternative the diluent. Transfer 5 µL of cell supernatant from the 96-well plate to a 384-small volume (SV) white detection plate . It is typically used to determine the concentration of nucleic acids and proteins. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Remove the plate sealer and read on an HTRF® compatible reader . Its. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Specialized filters allow for IR fluorescence. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. No washing steps are required. Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery (Fig. Lysates are then transferred to the assay plate for the detection of total-BTK by HTRF. Add. FOR HTRF CERTIFIED. FOR HTRF CERTIFIED. Make sure the setup for Eu 3+ Cryptate is used. FOR HTRF CERTIFIED READER. For more. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Plate 25 μL of cells in. Customer Transformation Hub. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. You can count on our plate readers to bring you the performance, reliability, consistent results, and the best user experience available—across a multitude of applications and a range of detection options. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. . 3 and higher include optimized reader settings, as well as data analysis, for faster results. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. PerkinElmer. BMG LABTECH’s UV-laser not only allows for faster read times in europium TRF assays, but it also greatly enhances performance in terbium-based TRF assays. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Dual monochromators for assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. It measures 96-well plates in less than 20 seconds and 1536-well formats in under one minute. It can also be further streamlined to TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. White 384-well low volume plate Make sure to use the set-up for Eu3+ Cryptate. EnVision Nexus ultimode Plate Reader 2 Benefits • Provides the speed and sensitivity to boost your lab’s productivity with ultra-high throughput • Freely configurable and upgradable to adapt to your needs today and tomorrow • Fully automatable for walkaway convenience • Certified for use with HTRF® technology Key featuresBMG LABTECH plate readers certified for LanthaScreen detection include the PHERAstar FSX, CLARIOstar Plus and VANTAstar. CLARIOstar is equipped with a specific optical device which enables the measurement of. For more information. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. 68. Read & analyse. • HTRF®-Certified Reader **. HTRF Signal + 2 hours @ RT [Cortisol] ng/ml 10 µL Standard or Sample 5 µL Cortisol acceptor reagent 5 µL Cortisol donor antibody ADD READ & ANALYSESafire 2™ readers must be appropriately be configured for HTRF readout by setting up the measurement conditions in the “multilabeling” function of Magellan software. Excitation lasers significantly improve performance and lower. If not, our scientific team will help you choose the instrument that best suits your needs and budget. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Read & analyse. They can be used in a no-transfer. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. and TR-FRET, for example to detect PerkinElmer’s DELFIA, HTRF and LANCE assay technologies Time resolved fluorescence certified for use with HTRF. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. HTRF is compatible with a large range of available microplate readers. FOR HTRF CERTIFIED READER. Read & analyse. FLUOstar Omega Filter-based multi-mode microplate reader . For more information about HTRF. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes• HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. FOR HTRF CERTIFIED READER For more. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. VICTOR® Nivo™ multimode plate reader 2 | For research use only. For HTRF certified reader For more information about HTRF® compatible readers and for set- up recommendations, please visit our website. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. All requiredThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for• ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. FOR HTRF CERTIFIED READER culture plate to a 384-well small volume white plate. PerkinElmer EnVision Multimode Plate Reader. HTRF KinEASE-TK kit and HTRF 96-well low volume plate were obtained from Cisbio Bioassays. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. FOR HTRF CERTIFIED READER. The VICTOR Nivo is a HTRF certified microplate reader. Lysates are then transferred to the assay plate for the detection of Total H2AX by HTRF ® reagents. 7ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. FOR HTRF CERTIFIED READER. $352. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. This protocol, HTS designed, allows. FOR HTRF CERTIFIED. FOR HTRF CERTIFIED READER. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. For more information. FOR HTRF CERTIFIED READER For. The signals are produced by, converted by, or transmitted through the. 1. FOR HTRF CERTIFIED READER. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. Plus . In particular, these parameters should be entered as defined in the table below Setup Top filter cube EX 330 / LUM (block #18) EM 620 / 665 / LUM (block #41) Light source LaserDetect flash, glow, Dual Luciferase ® Reporter and BRET assays. Provide more sensitive and precise results. The FLUOstar ® Omega is a multi-mode microplate reader with three detection modes. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Lysates are then transferred to the assay plate for the detection of phospho- CHK2 (Thr 68) by HTRF. Your lab might even have one already. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Its. Handle complex biological matrices easily. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYONE-PLATE ASSAY PROTOCOL: Small volume detection plate. * For HTRF microplate recommendations, please visit . ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. 1) . For more information. Data Analysis HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. We would like to show you a description here but the site won’t allow us. The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. For more information about HTRF. It can also be further streamlined to a one-. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). For more. These plates can be read on all HTRF certified readers, thus making their implementation in. For more information about HTRF. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The plate reader. Evaluation of the performance of the Spark™ multimode reader for far red fluorescence emission. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. For more information about set-up and compatible HTRF ® readers, please visit our website at: ® pre-mixed antibodies TWO- PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells . This protocol. Envision Nexus. 6% for 1,408 wells and a Z’ value of 0. This protocol gives the cells viability and confluence to be monitored. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Our assay development platform relies on PHERAstar readers for absorbance, fluorescence, luminescence, and homogeneous time resolved. Partnerships for delivering turn-key solutions. + 4 µL HTRF pre-mixed antibodies . Lysates are then transferred to the assay plate for the detection of total-Rb by HTRF. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. For more information. HTRF combines time-resolved fresh detection with FRET and is mainly used for drug screening in high-throughput. Your lab might even have one already. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 9. (2) Forskolin activates the adenylyl cyclase enzyme and increases the intracellular level of cAMP. Plate 25 μL of cells in. HTRF plate reader. Suggested plate map: 1: 2 3: A: 14 μL Std 0: 3 μL IP1 d2 Reagent 3 μL IP1 Tb Cryptate Antibody• HTRF®-Certified Reader. An. culture plate. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator. CLARIOstar is equipped with a specific optical device which enables the measurement of. Specialized filters allow for IR fluorescence. + 4 µL HTRF ® pre-mixed antibodies . For a list of HTRF-compatible readers and set -up recommendations, please visit. HTRF certified . Read & analyse. 1. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. For more information about HTRF. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. + 4 µL HTRF ® pre-mixed antibo dies 2. Do not pre-mix the d2 and Cryptate solutions prior to dispensing Make sure to use the set-up for Eu Cryptate. CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. Neocarzinostatin dose-response on Jurkat cells. 5 μL of Binding buffer 2X. These plates can be read on all HTRF certified readers, thus making their implementation in. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYDetection. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. EGFR protein (PV3872, 0. It also integrates with the. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. This protocol gives the cells viability and confluence to be monitored. S. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4 SpectraMax® M5 Multi-Mode Microplate Reader MLDVM5 SpectraMax® M5e Multi-Mode Microplate Reader (HTRF certified) MLDVM5E To prevent evaporation during assay incubation steps, we recommend the use of TopSeal-A PLUS, a clear adhesive seal for microplate. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Phospho-Rb (Ser780) two-plate assay protocol. • HTRF®-Certified Reader **. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate 25 µL cells + 5 µL compounds + 10 µL supplemented lysis buffer (4X)* + 4 µL HTRF® pre-mixed antibodies Read & analyse Incubate 4h at RT Transfer 16 µL supernatant LysisAdd ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified . Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. The ratio for each well must be calculated individually. The laser beam passes through the sample well. HTRF-specific. DATA REDUCTION & INTERPRETATION 1. For more information about HTRF. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Lyse cells Read on an HTRF compatible reader . • HTRF®-Certified Reader. For more information about HTRF. 5 µL. For more information about HTRF ®Seal the plate and incubate for Overnight. Plate 25 μL of cells in. Dual-Luciferase® (DLR™) gene assays. Read & analyse. Incubate overnight at RT. Lysates are then transferred to the assay plate for the detection of Total-eNOS by HTRF. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. Revvity. Incubate 2h at RT Transfer 16 µL supernatant. certified for use with HTRF. • Select Excitation Filter 320x40 HTRF Eu cryptate* • Select Excitation Optic Empty • Select Emission Filter 495xm10uv • Select Emission Optic 2 – FPThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates to a low volume detection plate (either HTRF 384-lv or 96-lv plate) before the addition of HTRF Phospho-Rb (Ser780) detection reagents. STANDARD . Fluorescence intensity – Fluorescence intensity is one of the most popular detection modes, and there are innumerable fluorophores available for applications such as DNA or protein quantitation, reporter-geneCertified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. EnVision Nexus ultimode Plate Reader 2 Benefits • Provides the speed and sensitivity to boost your lab’s productivity with ultra-high throughput • Freely configurable and upgradable to adapt to your needs today and tomorrow • Fully automatable for walkaway convenience • Certified for use with HTRF® technology Key features BMG LABTECH plate readers certified for LanthaScreen detection include the PHERAstar FSX, CLARIOstar Plus and VANTAstar. FOR HTRF CERTIFIED READERThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. n HTRF plate validated (QC with reference HTRF assays) n Low to high volumes (10 µL to 350 µL) n Can be used with all HTRF compatible readers n For cell-based or biochemical assays Trying is Believing! Plate Characteristics HTRF® 96 - well low volume plate CulturPlateTM 96 - well Microplate CulturPlate TM 384 - well Microplate ProxiPlateTM 384 - Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Incubate overnight at RT. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. It utilises an ultra-fast UV/vis spectrometer or filters for absorbance as well as highly sensitive filters for all other detection modes. The PHERAstar ® FSX and CLARIOstar ® microplate readers offer the best possible performance for AlphaScreen ®, AlphaLISA ®, and AlphaPlex™ assays. The dual measurement. Optimized instrument settings for the HTRF camps HiRange assay. This protocol gives the cells viability and confluence to be monitored. Seal the plate and. With its ease of use software and remote access from almost any device, it is ideal for multiuser environments. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Transcreener® ADP2 FP assay. The DLR assay is a two step reaction that uses two luciferase enzymes, Firefly and Renilla (Figure 1). FOR HTRF CERTIFIED READER . Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. You can verify your reader’s capacity to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. protocol with suspension cells or with a. It features a modular design that allows users to add or change detection cartridges according to their needs. It features a modular design that allows users to add or change detection cartridges according to their needs. For HTRF certified readerThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Read & analyse. Incubate 4h at RT. + 4 µL HTRF ® pre-mixed antibo dies 2. Plate 25 μL of cells in. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. FOR HTRF CERTIFIED. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Learn about the principles, applications, and benefits of HTRF in this Agilent application note. Microplate readers are only certified if they. Customer Transformation Hub. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. The plate was covered and incubated for one hour at room temperature. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes To prevent evaporation during assay incubation steps, we recommend the use of TopSeal-A PLUS, a clear adhesive seal for microplate. Incubate overnight at RT. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. 0. This protocol, HTS designed, allows miniaturization while. The CLARIOstar® Plus is our most flexible multi-mode plate reader. For more information about HTRF. SV detection plate. 4 mg/ml. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersHTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. These plates can be read on all HTRF certified readers, thus making their implementation in. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. Make sure the setup for Eu Cryptate is used. View Brochure. Incubate overnight at RT. no. Plate 25 μL of cells in. Microplate readerFluorescence microplate readers usually contain a light source, an optical system for the selection of excitation light (i. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. FOR HTRF CERTIFIED READER.