of 3 wells of a 96-well plate, from 1 of 3 independent experiments. UV-Star ® 96W Microplate, well working volume 25-370 μL; total well volume 392 μL. Plate chamber design of the SpectraMax category 1 microplate readers. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and. Microplate Spectrophotometers. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. 20 plates/hour (2-minute read time) 96-well 1,920 data points/hour 384-well 7,680 data points/hour 1536-well 30,720 data points/hourMicroplate Reader, For Lab. 96-well PCR plates are plastic, heat-resistant multi-well microplates designed for use inside PCR machines or real-time (qPCR) thermocyclers. #3693; n = 4 for each standard). Silica excitation fiber, glass emission fiber. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. A 1:10 dilution series of ATP was set up in solid white 96- and 384-well plates at concentrations from 10 uM down to 1 nM, along with blank wells containing no cells. You can mix the contents of the wells in a microplate automatically by shaking before each read cycle. The SpectraMax® L microplate reader measures flash and glow luminescence assays in both 96- and 384-well plates. Click “Next”, and the reader will start scanning the four corner wells; a progress indicator will appear: fter the reader finishes scanning the four corner wells, you will see a screen with an image of the upper left corner A well and a pink crosshair. A standard 96 well plate was set up as described in Protocol Table 4. The clear bottom plates offer the advantage of being able to examine the cells by microscopy during the course of the. x. minutes and begin reading microplates with seven mouse clicks. Read up to 2 wavelengths in endpoint absorbance for 96-well plates. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. 5 ng per well. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. The plate reader optics are tunable for. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. • High antibody-binding surface improves the overall performance in. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. This eliminates the need for standard curves, and 96–well: 3 mP 384–well: 3 mP 1536–well: 6 mP 1 LUMI Glow luminescence detection for different plates 0200-7014POS Glow for 96-well plates 96–well: 1 pM 96–well: 1. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Devices® SPECTRAmax 190 microplate reader at a wavelength of 600 nm. Other. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andthe iD5 reader’s filter system. Top or bottom detection is available with a simple click of a button. Step 2: Launch SoftMax Pro Software. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Fireflysci UV/VIS Fluorescence Validation Plate for Well Plate Reader (290-562nm) FLVP-01. Materials. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. The plate reader can measure multiple readouts, including UV and. x. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Click on the crosshair and move it so that it is centered over the well image. 1. 100 ˜L (96-well) or 25 ˜L (384-well) of Loading Buffer was added to each assay well. , Corning, NY) or Greiner. The system features 96, 384,. Tristar 3 Multimode Microplate Reader. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Uncontrolled Copy for Training and Reference Purposes Only . 1 - 32 of 54 Sort View Show > Well Plate Reader Accessory PerkinElmer For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro. • 0. Flexible liquid transfer Using an 8- or 16-channel pipettor, the FlexStation 3 Reader offers added assay flexibility over dispenser-based systems by transferring reagents from 96 or 384 distinct wells in a source plate to a read plate, simultaneously. 5 ng per well. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. Related products. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. Published Dec 16,. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. The EMax Endpoint ELISA Microplate Reader from Molecular Devices (one of the pioneers of microplate detection) provides a simple and durable dedicated endpoint detection method. The UV-Plate is compatible with the following plate readers: Bio-Tek PowerWave™ 200 Bio-Tek µQuant™ BioRad UltraMark™ Molecular Devices SPECTRAmax® 190, 250, 340PC, and Plus Ordering Information Cat. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Molecular Spectroscopy Consumables & Accessories Thermal Analysis. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. It features a modular design that allows users to add or change detection cartridges according to their needs. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ Microplate Washer. x. Reads various kinds of 96-wells plates, and is equipped with shaking function. Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. 96-, 384-, and 1536-well microplates. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. The plate was preincubated in the reader at 37°C for a minimum of 10 minutes. The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. The. The LysoTracker fluorescence at ex/em 574/594 was measured on a Spectramax i3x plate reader (Molecular Devices). Bowman Dispensers. . The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm) with 4 filter choices included at time of purchase. HeLa. Integrated stacker option. Type:Single Mode Absorbance Plate Formats:96 well plates Accuracy:0. Category 2 and 3 microplate readers use gradient heating as well. Microplate Readers. Flexibility to read a variety of plate formats - SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Optimized for fluorescence microplate readers. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. x. QC tools and IQOQ services. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm: Measurement Type: Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra: No. 2 pM 384–well: 3 pM 96–well: 2 pM 384–well: 4 pM 1 0200-7012POS Glow for 96-, 384-, and 1536-well plates Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. 9000-0756. 303 unable to cal dark current. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. M2. 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. A microplate adapter is required in the reading chamber drawer for 96- and 384-well assay plates. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. Expand Microplates and select the newly created plate. 0% Normal and Rapid modes, 96- and 384-well plates; Absorbance Repeatability: 0. The VICTOR Nivo. Turn the instrument around so that the back of the instrument is. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Service Contracts, Preventative Maintenance and. More Product Information. Molecular Devices: FlexStation 3: Multimodal: Limited - monochronometer based: N/A:the iD5 reader’s filter system. 96-well) while others have flexibility to. as well as all other Molecular Devices' microplate readers. They can be read by most microplate readers. 1 OD at 230 nm. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. The VersaMax reads 96-well microplates. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. It features fully-automated kinetic reads of the 96-well plate via the WinKQCL TM Software. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. Conventional technique: subjective, time-consuming. x. It has a 340-750nm Spectral Range. These models can no longer be repaired. We would like to show you a description here but the site won’t allow us. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. White 96-well platePlate formats. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. 96 wells 384 wells 1 pM 1 pM Optimized bottom sensitivity (fluorescein) 96 wells 384 wells 2 pM 2. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the. Molecular Devices' microplate readers. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Performing molecular and cellular analyses in multiwell microplate formats is an important methodology for high-throughput screening, or for boosting throughput generally. 190. Important things to consider when purchasing a multimode microplate reader. * Note: Programming the washer with a final aspirate height of 4. With theMolecular DevicesWe designed and fabricated different sized microfluidic covers, matched to the desired experimental conditions: Fig. a white 96-well half-area microplate (Corning cat. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. It offers extremely low absorbance background, excellent. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. 312 gain calibration failed. A = Log 10 (I 0 /I). Published Dec 16, 2020 | Updated Jun 22, 2023. Before dispensing reagents to the microplate, the VersaMax Reader was powered on and. 2 with 1000 cells. 90349). Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. Browse for different fluorescent readers below and compare across different instrument suppliers. com. The CellTiter-Glo assay was used to measure ATP. SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use: $20/hr: $32/hr. Products : Ionworks HT, Ionworks Quattro. Microplate readers compatible with 96-well plates Single- to multi-mode readers combine an absorbance plate reader , a luminescence plate reader , and a fluorescence plate. 2. SpectraMax i3x Multi-Mode Microplate Reader. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of the human AT1R receptor. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Thermo Scientific Pierce Streptavidin Coated Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. the use of a reader calibrated with primary NIST and NMI standards. Versa Max. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. BioTek Instruments Synergy Neo2 Multi-Mode Reader Fluorescence Measuring Equipment. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. 1. and the A1 corner of the plate adapter (Figure 1-2). Dark blue indicates well will be read. Well scanning With well scanning, multiple points within each well can be read, providing a high. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. 9. When the user applies a set temperature of 37°C, the top is heated to 37. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. To validate the assays’ ability to detect a wide range of viable cell numbers, a series of cell densities was set up. Plate types Flat and round 96-well plates Data output Export to PC Detection system Single channel silicon photodiode Reading speed 25 seconds Software SoftMax Pro Software Shaking None Temperature control None Linearity 0. 96-well plates - Filter plates with 0. 00 . Plus 384. Capabilities. The reduced cost of the device represents the major technical contribution of this manuscript, as costs were reduced 7 fold from previous reports. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. % transmittance of plates used in a plate reader. 384-WELL SBS STERILE MICROPLATE. Learn how you can utilize microplate readers to drive landmark discoveries. x. Products : SpectraMax L reader How long does it take to dispense into a 96 well plate with SpectraMax L reader injection? For a 96-well plate it takes a total of 27. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data. Fluorescence Microplate Reader Comparison Chart. Robust shaking and consistent temperature control optimize bacteria and yeast cell growth. Molecular Devices (UK) Ltd. SpectraMax® Microplate Reader Suite of Products Knowledge Base. Packed 25 plates per sleeve, 2 sleeves per case. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). When a. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. to each well. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. 00 USD. . UV absorbance detection. 00. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. 97 independent and fixed optical fibers can achieve a mean time between failures (MTBF) of 20,000 hours, which keeps the microwell plate still during the entire reading cycle,. #SpectraMax L Config) Methods. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. 7°C (gradient heating design). Thermomax model provides thermal control and vertical shake, perfect for. Look for a washer which can handle a variety of plate types, including deep well plates and 96-, 384- and 1536-well plates. Temperature variation ± 0. Important things to consider when purchasing a multimode microplate reader. Two different agonists, acetylcholine and carbachol, were tested. QuickSync. 000 to 2. Accurate, efficient nucleic acid quantitation is critical for molecular biology labs that work with precious, rare, or difficult-to-process samples and applications requiring precise measurement. 25, 3. Intuitive graphical user interface makes each assay extremely easy to set. White 96-well plate Plate formats. View Product. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. SoftMax Pro Software minimizes setup time for detection and automates standard curve fitting and sample quantitation. Published Dec 16, 2020 | Updated Dec 16, 2020. Highly selective for dsDNA over ssDNA or RNA. Washer maintenance kit: 2 X T 2. The StakMax stacker also allows users to feed plates into the loading area manually. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. Microplates, also known as microtiter plates, are rectangular multi-well plates used in a variety of assays from ELISA to PCR. 000 – 4. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. Bioz Stars score: 86/100, based on 40 PubMed citations. Elevated well walls for easier sealing Round bottom formats Square and round well formats Conform to SBS standards Lids and cap mats available Packed in sleeves of 6, 24. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). Description Bottom Volume Qty/Pk Qty/Cs 3635 96 Well UV-Plate Flat 370 µL 2550per well of this semi-solid mixture was pipetted into 6-well plates. 24-, 96-, 384-well plates and microwell strips; Manifold Channels: 96-well washing: 8-well (1x8) manifold, 2x8-well manifold, 12-well (1x12) manifold, 8-well short tube (1x8) manifold. A microplate adapter is required in the reading chamber drawer for 96- and 384-well assay plates. 0% ± 0. 96 wells 384 wells 1 pM 1 pM Optimized bottom sensitivity (fluorescein) 96 wells 384 wells 2 pM 2. Three kits covering a wide range of dsDNA concentrations. the Gemini XPS reader. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. The VersaMax Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible. 5032338. For the name of the nearest authorized Molecular Devices Corporation service facility please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Service parts for these models are not being produced and are no longer in stock. Plate Shaking. 0200-6263. 190. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SUPPORTED PLATES Microplates having 6, 12, 24, 48, 96, and. Including injection time, it takes approximately 68 secs. Molecular Devices: EMax Plus: SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise:. A 1:10 dilution series of ATP was set up in solid white 96- and 384-well plates at concentrations from 10 uM down to 1 nM, along with blank wells containing no cells. These robust readers have been placed in labs from. 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. • Specially formulated optically clear polystyrene and treated to provide protein coating capacity. Working in. Well-to-WellPrecision RFULinearity RelativeFluorescenceUnit(RFU)ScaleRatio(GeminiEM,XPS,SpectraMaxM2-M5e, FlexStation3only). UT-6550 Microplate Reader is a reliable and robust instrument for a wide. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. The ImageXpress® Pico Automated Cell Imaging System is more than a digital microscope, combining high-resolution imaging with powerful analysis. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. 0025 OD from 0. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Absorbance equation. e 3370 Costar 96 Well. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. These plasticware can come in a variety of formats, such as skirted, semi-skirted, unskirted, clear, colored, and barcoded. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. xThe SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 96-, 384-, and 1536-well microplates. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the assay. Quickly image a variety of plate formats and cell types - Image a 96-well plate in under two minutes. 0 . 1 x plates; Instrument dimensions. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. White plates reflect the light, enabling even low levels of Luminescence to be detected. Both the P and M injectors were set to deliver 100 µL (96-well plate) or 25 µL (384-well plate), with a 2-second delay and 10-second integration time. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro Software. Description. Dear Nelly, In a micro plate the fluorescence emission optics are very different from those in a single cell because of obstruction, reabsorption etc. maximum-illumination-channels. TNF standard curve. x. Page 1 of 8 . Contents of wells were pipetted up and down several times to mix. The opaque walls prevent stray light from being detected from neighbouring. Usage/Application: Lab. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. 313 reference gain check fail. 0001 ABS (calculated) • Light source: Halogen lamp, 8V/50W • Wavelength range: 405, 450, 492, 630 nm, 4 more filters optional. Microplates, cuvettes (via adapter) Detectors. #CA961) Methods. The instrument reads any standard cuvette, microcuvette, test tube and 96 well or UV transparent microplate over full 190-1000 nm wavelength range. Features. x. Compare Tool. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. and glow luminescence modes in 96- to 384-well plates. Controlled by the computer. These lab workhorses are used in drug discovery. Answer. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Microplates, cuvettes (via adapter) Detectors. 1c shows a cover designed to perfuse 8 wells in series on a standard 96-well microplate, as well as a cover for the entire standard 96-well microplate. Washers configured for both 96- and 384-well plates. Dimensions: 18. x. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. To test the optical clarity of the various microplates at low wavelengths, one well of each plate was filled with 100 μL (96-well) or 20 μL (384-well) distilled water, and its. 46Click the Read button in the SoftMax Pro3 reader combines Molecular Devices SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Products Clone Screening Accessories and Consumables Deep-well Plates Deep-well Plates Deep well blocks available in 24, 96 and 384-well formats Product Options. In SoftMax Pro, Dual Read mode was selected. A = 2 - log 10 (%T). The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. A polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. 5 OD at 492 nm Computer interface USB 2. #iD5-STD). 96-, 384-, and 1536-well microplates. • Photometric range: 0. Never order or replace filters to optimize an assay again!6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. Molecular Devices LLC Applications: Inquire; Plate Formats: 96-, 384- well. Published Dec 16, 2020 | Updated Nov 07, 2022. Quartz Plate found in: Thick Quartz Ground and Polished Plates, Solid Quartz 96 Well Clear Plate with Clear Base, Quartz Parallel Plate Probes for DMA 7. Consequently, FLIPR has been used. Kinetic readings were started immediately with a Molecular Devices FlexStation Plate Reader. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. x. D. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Products : ClonePix 2 System, QPix 450/460 Systems, QPix 420 System, CloneSelect Imager. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. Click SoftMax Pro Software User Guide. These robust readers have been placed in labs from Antarctica to. Dual injectors for kinetic assays. softmax pro software, gemini em reader, molecular devices stakmax microplate stacker, softmax pro data analysis, spectrofluorometer, monochromator microplate reader, cell migration assays, cell. 4 (Molecular Devices cat. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Time-resolved phosphorescence. . #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. With various on-board features and capabilities, the ThermoMax. Fluorescence Microplate Reader Comparison Chart. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. It also integrates with the powerful SoftMax Pro Software for. Microplate readers, SpectraMax®, with SpectraDrop. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 0. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Find our technical support information along with contact details. The VersaMax Microplate Reader is supplied with SoftMax® Pro Data Acquistion & Analysis Software, Molecular Devices' industry leading all-in-one data acquisition and analysis. Sensitive luminometer with programmable injector options for 96- and 384-well microplates. Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. This is a Molecular Devices Thermomax 96 well Absorbance Microplate Readers. com Molecular Devices (Germany) GmbH ismarcring ierach an der iss erman ©2019 Molecular Devices, LLC. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Self-righting base, up to 40 degrees past center without dispenser. Anticancer drug assay was also conducted using conventional 96-well plates as controls. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). A 96-well microplate reader for absorption spectroscopy was designed, constructed, and tested at a total cost of ca. REF (SpectraMax. #CA961) Methods. We have the following plate-reader instrument available for on-site screens and other projects Molecular Devices Spectramax Paradigm for luminescence, fluorescence, and. Next-generation luminescence plate reader. What are the dimensions of the plate? Code. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Running both standard spectrophotometer and microplate. A custom 96-well plastic (polyethylene terephthalate/PET) plate is attached via epoxy to the mounted CMOS ICs, designed with a standard form factor to be compatible with high-throughput. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise. Or scan up to 384 wells at any time to determine the best assay conditions. 2. Versa Max. 00 Add to cart; Molecular Devices EMax Microplate Reader $. one 96-well sample plate can be controlled from 15–40°C,. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. Unparalleled performance on a personalized platform. 315 can't find zero order. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. All readings were taken in clear, polystyrene 96-shallow-well, flat-bottom plates.