For GPC columns, remove column from the flow path so nothing is accidently flushed into the column. Operable in constant flow or constant pressure mode, as well as isocratic, low pressure gradient, ternary high-pressure gradient, or quaternary low. Vent. 23 MPa. The auto purge function was achieved by devising the mechanism of flow lines including the high-pressure 6-way valve and by modifying the solvent delivery unit check valve. 0*Today, we're diving into the exciting world of lab consumables and equipment, specifically focusing on the Shimadzu HPLC pump. Replace piston seals. When they fill with air, they lose their prime and stop • Effects Column Life pumping [26]. The system pressure and PreColumn pressure also fluctuates between 0. This technique is commonly used in labs for the purpose of separating, identifying, and quantifying components in a mixture. Purge and condition lines A1, B1 using pathway 1, then A2, B2 using pathway 2 using a 50:50 composition to ensure both pumps are taking and delivering the same volume of HPLC flushing solvent. This video shows the operation a HPLC purge valve. Pump 100% isopropanol through the pump for 3 minutes. Purge the pumps each time fresh eluent or water is prepared. Purge Solvents a. 06% or 0. 4. Delivering Scientific Insights to Improve Human Health and Well-Being. 3 Page 5 Column Observations Potential Problems[][]Purge the pump with fresh diluent (filtered and degassed). It is by far the number one cause of problems in HPLC. off. 05 0 0. Close the purge valve. Priming and Purging the 1525 Binary HPLC Pump. RCM in the Quat Pump module i. Make sure that the AVR7-3 inject valve is in the Purge position. 물론 이건 내 생각이다! 여튼 purge. 물론 이건 내 생각이다! 여튼 purge. Solving Problems That Matter - Bioprocessing. 2 μm filters. Make sure that you know which solvent is linked to which pump. Documents & Downloads. Then press “Enter”, “1”, “Enter”, “CE”, “CE”. After purging is done click [Pump on] button / to deliver the solvent for at least 20 to 30min to rinse the flow line . 2. Next, close the vent valve to slightly pressurize the gas in contact with the liquid. When they fill with air, they lose their prime and stop • Effects Column Life pumping [26]. If [Auto Purge] is not set in the method use the manual purge option by opening the drain valve and pressing the [Purge] button on the instrument. SPE, LLE) on. 17: Ready Check Results window appear s after the NC pump blocks purge is executed 2. High Pressure Liquid Chromatography. If you have a pump with an automatic purge valve, read the note below the article. This is needed to purge any air from the tubing, introduce fresh mobile phase to each line and then to VERIFY that each channel delivers the reported amount of fluid to the column (measure it). • Purge each channel for at least 10 min at 5 mL/min. Almost all descriptions in the manual apply to all pump models in the LPG-3000 pump series and cover both the standard (stainless steel) and biocompatible pumps. (Most ternary pumps actually have four solvent reservoirs. Using an autosampler reduces manual labor and improves injection precision and accuracy compared to manual sample injection, an absolute priority for a quantitative HPLC analysis. 2 Content 4 2. HPLC maintenance tutorial on how to change the High Pressure Filter on an Agilent 1290 G4220x or G4204x Pump. ), prime system if necessary. , 5-10 mL/min. In other words is the system configuration comprising of the pump, injector/autosampler, column, and detector is appropriate for carrying out the analysis in. 5 01. A purge valve with The pump assembly includes a pump head with a passive inlet valve and an outlet valve. Operation of DAD: [][]Switch ‘ON’ the power of Quaternary Pump, Autosampler, Thermostatic Column Compartment, Detector, CPU, Monitor if already not switched on. Weigh the sample into a syringe. The system pressure and PreColumn pressure also fluctuates between 0. If only an occasional bubble is present, the flow rate will be erratic, causing retention time problems. Solvent Degasser – removes air gases from the solvents as they flow to the HPLC pump. The workstation pumps will run at a default flow rate of 10 ml/min and the indicator light will flash green. A damping unit is connected between the two plunger chambers. Their pulse-free solvent flow, from analytical to semi-preparative flow rates, makes them ideal for use with highly sensitive detectors. Pump 100% isopropanol through the pump for 3 minutes. Open the black purge valve on the front of the quaternary pump by turning it several times counterclockwise. GasesUltiMate 3000 Series: Pump Series Operating Instructions Page I Declaration of Conformity (Original Declaration) Product: UltiMate 3000 Series Pump Types: LPG-3400AB, LPG-3400M and LPG-3400MB DGP-3600A, DGP-3600AB,. This pump enables quaternary solvent delivery at pressures up to 800. But we managed to stabilize it by washing the system overnight with acetonitrile. Note: A wet connection between the tubing and the column avoids any air bubbles being trapped during. The dual piston design has minimal pulsation for stable baselines. We have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the pump head. Maintaining Your Agilent 1100 Series HPLC System Optimize performance and maximize efficiency in your lab. Change the pump program back to 50/50 water/ACN to re-equilibrate the system e. • Flush the entire system flow path with water for at least 10 min at 2 mL/min. From my little experience i learnt that it may be due to air trapped in. 1. 0Mb)Page 35: Purging The Pump The pump should never be used for priming/purging empty tubings (never let the pump N O T E run dry). HPLC is an abbreviation for High Performance Liquid Chromatography. the solvent flow of the HPLC If equipped with a ballast valve check that the ballast valve on the rough pump is . For more information, please visit Connect the Restriction capillary (5022- 2159) to the purge valve. After purging is done click [Pump on] button / to deliver the solvent for at least 20 to 30min to rinse the flow line . Click on Method 1. Properly shut down all the HPLC modules from the front (LC-20 and LC-30) or backUltiMate 3000 Series: SD, RS, BM, and BX Pumps Operating Instructions Page I Declaration of Conformity (Original Declaration of Conformity) Product: Thermo Scientific Dionex UltiMate 3000 - Pump Types: ISO-3100SD, ISO-3100BMThe PTFE frits in your Agilent LC pumps will need to be replaced from time to time as part of a routine maintenance program. g. The Breeze software has a Purge wizard that lets you purge the pump, 717plus Autosampler, and a 2414 Differential Refractometer. More lab efficiency with your auto delivery schedule, modify and cancel it. Focus on the column's inlet: After the initial flush, reduce the flow rate to 1 mL/min or so and close the purge valve until no more bubbles emerge. You may use the. Pump Types LPG pump type Eluent composition at the low pressure side => Before the pump head Eluent is composed through proportioning valves Eluent segments pass working and equilibration cylinder Up to four solvents can be mixed HPG pump type Eluent composition at the high pressure side => Behind the pump head UltiMate 3000 Series: Pump Series Operating Instructions Page I Declaration of Conformity (Original Declaration) Product: UltiMate 3000 Series Pump Types: LPG-3400AB, LPG-3400M and LPG-3400MB For stainless steel flow paths, proceed to Step 6; For PEEK flow paths, the cleaning procedure is completed. Afterwards. If your problem is. Residues or contaminations may block filters or capillaries. Pump – Open the purge valve and purge every solvent channel with 5 mL/min for 5 minutes to remove bubbles in your solvent lines (Note: if the backpressure at purging is higher than 10 bar, please replace the PTFE frit in the purge valve) – Make sure that the flushing solvent is mixable with the storage solvent of your solvent channel. The reduction in condensation risk can allow the pump to achieve its vapor duty at. Required parts: New pump head; Spacing tool; Size 6 hexagonal wrench; ¼” wrench; Preparation steps: Flush the pump head with an appropriate solvent using the purge function Next, it purges the flow lines up to the column with the mobile phase at the starting gradient concentration and then pumps it through the column at a low flow rate to protect the column. Purge pump at high flow rate (e. The good news is that there is a simple solution: open the purge valve, turn the flow rate to five mLs a minute, let it sit there for 10 to. 3601-EN-5. And, remember, if you have a dual-piston pump, you have two piston seals to replace. Before using an HPLC system, you should prime all of the lines in your HPLC pump. To manually purge the reference side of the 2414 flow cell, press the Shift + 1 button (Purge) on the screen while the pump is flowing mobile phase. Monitoring your HPLC system for potential issues can help to. e. Plot the graph of Injection volume against area. Frit, PTFE, 5/pk Add to Favorites + Create New list Item successfully added to your list Subscribe to this item in cart or checkout. the user. 19 Section 3: Pump Maintenance 20 Purge Valve 21 Outlet Ball Valve 21 Active Inlet Valve (AIV) 22 Seals and Pistons 25 Pump Head 26 Parts and Supplies 27 Solvent Inlet FiltersChromaster HPLC 5110 pump with auto-purge valve. The 1260 Infinity II Quaternary Pump combines proven reliability with highest flexibility in automated. ), prime system if necessary. HPLC is an abbreviation for High Performance Liquid Chromatography. Run each pump for 2 minutes. Seat backfl ush is enabled by a second pump, an integrated high-pressure fl ush pump. Make sure that you know which solvent is linked to which pump. 1. Click the [Auto Purge] button to perform auto purge. 2. close the purge. L/mni for 5mni . Some pumps will require to manually open the purge valve and in others it is done via the software. DO NOT PURGE THE SYSTEM WITH THE BALL VALVE CLOSED! THIS WILL RESULT IN PRESSURE TOO HIGH FOR THE PUMP TO HANDLE AND CAN RUIN THE COLUMN!!! 6. (2) Dry Priming using the HPLC pump to draw the mobile phase out of the bottles, through the lines, degasser channels and to the pump head or multichannel valve. When I used the pump C last week the pressure suddenly fell down from 300 PSI to 0 (with 100% methanol, 0. Try adding acid or base to the wash solvent to see if the carryover is improved. Retention. Algae Growth in HPLC Systems 54 Prevent Blocking of Solvent Filters 55 Normal Phase Applications 57 4 Optimizing Performance 59. 19 Section 3: Pump Maintenance 20 Purge Valve 21 Outlet Ball Valve 21 Active Inlet Valve (AIV) 22 Seals and Pistons 25 Pump Head 26 Parts and Supplies 27 Solvent Inlet FiltersLC-40D XR Specifications. Operating principle. Remove and disassemble the pump head. Press the purge buttons again to. Maintaining the Purge Valve 139 Maintaining the Pump Head Assembly 140 Procedure 1: Pump Head with old Plunger Housing 141 Stage 1: Removing the Pump Head Assembly 141. If the column was used with buffered. Purge pump at high flow rate (e. During step 1, check that the solvent is freely moving through the solvent lines. Mass spectrometry, chromatography, spectroscopy, software, dissolution, sample handling and vacuum technologies coursesRemedy. It can mainly divided by two types 1. 45 or 0. If a column shows a pressure increase in a short period of use (small number of injections), there is usually an underlying problem. Fig. Figure 6: Pump head with purge valve and outlet to autosampler. A = 100%). Replace the pump head if the wetted surface is damaged, resulting in internal leaks compromising pump performance or if the pressure sensor is faulty. Each channel comprises a pump assembly including pump drive, pump head, active inlet valve with replaceable cartridge, and outlet valve. It offers gradient formation from up to four solvents at pressures up to 1300 bar and flow rates up to 5 mL/min. Initiate solvent flow by pushing the “PUMP ON/OFF” button on the front of the pump, then press “ENTER”. Easy to use fraction collector and Versatile Liquid Handler. General Recommendations For effi cient reduction of carryover, it is important that the sample be soluble in weak and strong wash solvents. Evaluate the results; Stop the batch; Apply a corrective pump purge; Re-equilibrate the system; Restart the batch Nexera looks for evidence of flow. 5. The pump inlet port is closed to allow the pump to run with the gas ballast valve in the open position, which helps to purge the pump. g. Treatment 1: Open a purge valve. • Use less-vigorous purging • Prepare fresh mobile phase • Check pump. From the data analysis program vent the system. Vanquish Binary Pump F: Up to 1000 bar (15,000 psi) and 8 mL/min. Check for flow. Maintaining your LC columns. Pump is compatible with HPLC and UHPLC, handling 2. A peristaltic pump (piezo pump) delivers fresh wash solvents to the fl ush port. In general, the degasser should be left on, and all solvent lines including the unused ones should be filled with solvents. Catalog number: 5040. Open the "drain" on LC-20AD. Purge the pump, check general functionality (for instance with a diagnostics test), read pre-compression value from pump panel and compare against values from manual (Thermo Scientific Dionex pumps only). 5. • Mast kit with mixer, purge valves and manual injector • Pre-formed 0. After the pump, the mobile phase encounters the autosampler. (Prime each pump head separately. If the preceding step does not solve the issue, remove the purge valve and start the pump. 05 min is normal however the behavior of the method should be used to determine what is a normal variation. 1, 3, and 4. The sensor signals the control board when the vacuum is below a preset level. DO NOT PUMP ANY FLUSHING SOLVENT THROUGH THE SEAL WASH LINE, OR INJECTION NEEDLE LINES. Autosampler – draws samples from vials and injects them into the solvent flow provided by the pump. HPLC Pumps. eluent through the pump for purging. For a system in reversed phase -> Purge all lines with hot HPLC-grade water (60 to 70 °C) to remove salts or ACN polymeric by-products. In such case clogging of the column is most likely happening due to: additive (i. From my little experience i learnt that it may be due to air trapped in. 2 Low Pressure No/Low Pressure Reading Question Yes No 1. Solvent Degasser – removes air gases from the solvents as they flow to the HPLC pump. Remove and clean pistons. 1) Locate the pump draw-off valce (handle located on front of the pump) and priming syringe. High Priced Liquid Chromatography. then you have a little bit of work to do to really prime the line as that pump is not really powerful enough to draw in liquid if it is super dry. Label bottles correctly with bottle content, and filling date / expiry date. Please refer to the section “Exchanging the Purge Valve Frit or the Purge Valve” on page 126 in this manual. A pressure sensor monitors the pump pressure. HPLC maintenance tutorial on how to remove the pump head on an 1100/1200/1260 Agilent HPLC. Purge pump at high flow rate (e. 8. Operable in constant flow or constant. 5 kgfcm -2, for example) for 10 to 30 minutes to purge the air from the mobile phase. Purging the Pump The quaternary pump will be purged by opening the purge valve. Close prime/purge valve. • Purge each channe lwtih 25–3m. Vacuum degassing: the solvent is exposed to a vacuum and the reduced pressure removes more than 60% of the dissolved air. The Logi-CHROM pump is compact yet powerful featuring a high quality HPLC pumping system. KB003532: What is the procedure to purge / prime a quaternary pump? Purging the Pump The quaternary pump will be purged by opening the purge valve. Click the [Auto Purge] button to perform auto purge. At this point, the mobile phase is ready for analysis. 11 Click everything on. 8 Click pump on the flow diagram and change the flow path into A2 and B2. This will redirect the solvent flow to the waste exit of the valve instead of passing through the whole system. Algae Growth in HPLC Systems 81 Prevent Blocking of Solvent Filters 82 Normal Phase Applications 83 4 Optimizing Performance 85 Using the Degasser 86. Count on the Arc HPLC System for high-efficiency separations and quality data to help meet your regulatory requirements with confidence. Then wash them out with water and re-assemble them on the pump head. First statement: Don’t let it run dry. Prepare the Pump • Use fresh or different mobile phase (as required). The pump assembly includes a pump head with an active inlet valve which has an replaceable cartridge, and an outlet valve. This small piece is meant to protect the rest of the system from particulates, and it needs to be replaced regularly. 1:55. e. Push the “PURGE” button on the front of the pump. Waters Student Academy 2023. It also includes relevant guidance for practical handling and mainte-(1:27min) Exchanging the Purge Valve Frit or the Purge Valve (3. HPLC maintenance tutorial on how to change the High Pressure Filter on an Agilent. • Press “Menu” till purge appears on screen. Agilent recommends a purge of at least 30mL in total for the 1200 systems. September 2, 2013 . Always do this one channel at a time (e. Related: 6. pump program to 90/10 water/ACN to flush the column for 15 min at 1. Once the system is purged, set the flow rate at 0. don't forget checking the purge valves. An optional seal wash function is available for applications using Contacting Us Vanquish Pumps (VH-P10) Operating Manual Page 3 Contacting Us There are several ways to contact us: Ordering Information For ordering information or sales support for HPLC products, contact Solving Problems That Matter - Batteries. ) If system has check valve, loosen valve to allow air to escape. 1 Turn on LC20AD (component 4) and SIL20A (component 3). Request Information. The name of the game in HPLC is repeatability. 3. UltiMate 3000 Series: SD, RS, BM, and BX Pumps Page II Operating Instructions. 2. Left pump connected to Vector PC post column derivatization. It provides HPLC systems with an automatic, continuous method of removing dissolved gases from mobile phases. L/mni for 5mni . Therefore knowing how to identify it and fix it is essential to developing technical mastery in HPLC. It is Liquid Chromatography. Some bubbles may be generated from the suction filter head dipping in the mobile phase. It is modified from of gas chromatography, it is applicable for both Volatile as well as Non volatile compound. the solvent flow of the HPLC If equipped with a ballast valve check that the ballast valve on the rough pump is . Turn off the pump by pressing "Pump" on the LC-20AD 4. Prime the HPLC pumps under the following conditions: - starting the pumps for the first time after it has been idle for more than 24 hours. This purging is carried out for several hours or overnight, depending on the system's size and the degree of contamination. We have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the pump head. Enter terms to search videos. 4. If the pressure does not rise, the purge valve needs to be replaced as it was the cause of high pressure. The degasser is a standard feature of the 1525µ Binary HPLC Pump and an optional feature of the 1525 analytical and 1525 EF Binary HPLC Pumps. A pump influence is indicated by baseline fluctuations synchronous with the pump cycle. Moreover, if the faulty purge valve completely blocks the engine, it cannot start or run. UltiMate 3000 Series: Pump Series Operating Instructions Page I Declaration of Conformity (Original Declaration) Product: UltiMate 3000 Series Pump Types: LPG-3400AB, LPG-3400M and LPG-3400MB DGP-3600A, DGP-3600AB,. The pump assembly includes a pump head with an active inlet valve which has an replaceable cartridge, and an outlet valve. However, in order to obtain a full understanding of the pump, Dionex recommends that you review the manual thoroughly before beginning operation. Email Priming and Purging a 1525 Binary Series HPLC Pump to a friend ; Read More. (You can press the same bottoms again to stop purging. (2) Dry Priming using the HPLC pump to draw the mobile phase out of the bottles, through the lines, degasser channels and to the pump head or multichannel valve. 1 Overview of HPLC. Plunger indicator rods Show position of each pump head plunger. Between samples when your compound is very sticky (apolar) to help clean the column. Properly shut down all the HPLC modules from the front (LC-20 and LC-30) or backThe 1260 Infinity II Preparative Binary Pump is an affordable high-pressure gradient pump for LC purification. Figure 2 shows how to perform the purge for an i-Series. Peak shape C. 2 1. Replacing a Check Valve Cartridge. Page 103 • Operation Figure 29: Dwell volume. Purge valve Purge functionFig. Before using an HPLC system, you should prime all of the lines in your HPLC pump. 6. Mass spectrometry, chromatography, spectroscopy, software, dissolution, sample handling and vacuum technologies courses. Close the purge valve, set the flow to a value that gives a pressure of 350 bar. if the column gets blocked or leaks occur † Automated electromagnetic purge valve for convenient system setup Agilent 1200 Series fraction collectors Designed for lowest delay volumes to minimize peak dispersion and carryover. Press “Prime” button (or pump at max flow), continue to draw on syringe until no bubbles are seen. Typically a 0. HPLC system. 6. This is needed to purge any air from the tubing, introduce fresh mobile phase to. Flow anomalies can interfere with the chromatography and stand in the way of repeatable results. Second, the dissolved gasses must diffuse through the membrane (flat film or tubing wall). 9 Pump Types LPG pump type Eluent composition at the low pressure side => Before the pump head Eluent is composed through proportioning valves Eluent segments pass working and equilibration cylinder Up to four solvents can be mixed HPG pump type Eluent composition at the high pressure side => Behind the pump head Pure solvents pass. , 5-10 mL/min. 4411-EN Revision 2. g. Vacuum degassing removes more than 60% of dissolved air. 1290 Infinity II High Speed Pump User Manual. Abstract and Figures. Among the various technologies developed for chromatography,. Open the purge valve and set the flow rate of 5 mL/min for each channel with HPLC grade water. United States. This pump delivers reproducible gradients for routine HPLC or UHPLC applications with UV or MS detection where speed, resolution, and uncompromised data quality are essential. Then wash them out with water and re-assemble them on the pump head. Wide peaks are generated either by substantial influence on the part of the HPLC system (bad capillary connections, void volumes, too large detector cells or ill. 01. 0 hours and also use 50% Methanol after water flushing. 2:32. 1 and purge the pumps for 5 min at a flow rate of 5 ml/min. Steps to follow: Store the column in an appropriate solvent (check column information sheet) When you remove the column, add a column union so the detector gets flushed as well in the following steps. Prime the pump with degassed solvent. Otherwise, locate where the increased pressure is coming from. c. Maintenance Kits for UltiMate™ 3000 Pumps. The manual covers the overview, installation, operation, and optimization of the pump, as well as troubleshooting and maintenance tips. Basic HPLC System Components. 15 mL/min. Prime the HPLC pumps under the following conditions: - starting the pumps for the first time after it has been idle for more than 24 hours. See Reference 1 for interactive video. If you've ever wondered how to purge this piece of equipment, you're in the right place. First, dissolved gasses in the mobile phase must diffuse to the surface of the membrane or film. That could help to reduce air bubble generation. Load a Method a. Optional solvent selection valves can be added for solvent. HPLC pumps are designed to pump liquid, not – Improve Peak Shape for Ionizable Compounds air. 02-0. This pump operates at pressures up to 600 bar and flow rates up to 10 mL/min. Purge pump and attempt to clean check valves. 1–1% solution by volume is sufficient. It is Liquid Chromatography. Press the Purge buttons A then B on the front of the workstation. 7. Purge the Pump (whenever changing buffers) a. "Chromatography" is a technique for separation, "chromatogram" is the result of chromatography, and "chromatograph" is the instrument used to conduct chromatography. For best performance you must know your eluents 2. 2 Wellplate Autosampler Purge Kit Features Features The new Wellplate Autosampler Purge Kit offers the following features: • Fits for Agilent 1100 Series Wellplate Autosampler (G1367A and G1368A (thermostatted)). Learn essential HPLC maintenance tips. If using MassLynx or Empower software, refer to the PDFs entitled Priming the 1525 Binary HPLC Pump Using MassLynx or Priming the 1525 Binary HPLC Pump Using Empower. 액상미립자측정기에서의 purge는 측정완료된 샘플용액을 제거하기 위해 사용하는 것 같다. try to locate source of particles (sample, eluents, pump mechanics, injection valve). Among the various technologies developed for chromatography,. Run at high flow: Next, Dolan advises to run the pump for several minutes at high flow (e. g. Check if application conditions are within the column specifications (e. Purge pump at high flow rate (e. Turn pump to ‘On’ 7. The 1525 and 1525 with EF heads are integrated, high-pressure, binary HPLC pumps that feature on-board pulse dampening and efficient mixing. If problem persists, flush system with 100% methanol or isopropanol. Treatment 2: Open the compression fitting at the top of the outlet check valve with a slow pump flow until solvent leaks around the fitting. Rebuilding the Reference Valve. 9-0. Press the EDIT/ ENTER key. Purging air from pump system: Change solvent line to a bottle containing 100% isopropanol. Priming and Purging the 1525 Binary HPLC Pump. For more information, visit tutorial demonstrates how to purge the system with Bio-Rad's new NGC liquid chromatography. From my little experience i learnt that it may be due to air trapped in. 1. Pump 15 minutes at this pressure to wear in the seals. 6. Unit conversion calculator for converting commonly used unitsSJ: There are two diffusion paths involved in inline degassing (ignoring vacuum side diffusion). 1290 Infinity II High Speed Pump. The following diagrams illustrate the operating principle of a modern HPLC pump: an electric motor drives a piston (generally made of sapphire or ceramic material) in and out of metal, ceramic, or PEEK piston chamber. Blocked channels can often be released by purging with a high flow with warm water (60 to 70 °C, HPLC grade). Use only HPLC-grade solvents and water filtered through 0. Introduction 4 1. † Superior quaternary mixing and a delay volume of 350 μL enable shortest run times, highest precision, and accuracy. some time this may be due to filters in the channels; please remove your filters (a inlet filter from your solvent reservoir bottle to the HPLC pump ) and sonicate in the Methanol. Perform flow rate check. Maintaining Your Agilent 1100 Series HPLC System Optimize performance and maximize efficiency in your lab. See your pump modules user manual for detailed instructions on re-assembly. Initially, the pressure was unstable. The manual. Make sure to confirm that the purge valve isHow to prevent your HPLC from running dry. you will need to • General cleaning Daily first re-prime the pump by purging the air from all the lines. Use the keypad to type in the solvent composition for pumps A, B, C, and D. Irreproducible gradient. Greetings. Fig 2. Check for leaks and perform PM. Equal functions for Right Pump found just below. 1. A key element of its user-friendly functionality is the accessibility of the. 8.