For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. A Costar 24-well tissue culture plate with col1 = blanks and doubling cell a list of plates specifically supported shows, 96, 384, 24, 12, 6 well types. All readings were taken in clear, polystyrene 96-shallow-well, flat-bottom plates. 3 OD at 200 nm. $488. Optional 16-way manifold. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. Published Dec 16, 2020 | Updated Jun 22, 2023. Meanwhile, Cowan discusses the importance of considering plate well densities and the angle from which microplates are read inside the reader: “You should definitely check the range of plate well densities supported by your candidate readers. Features. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. SoftMax Pro GxP Software version 5. UV-Star ® 96W Microplate, well working volume 25-370 μL; total well volume 392 μL. 5in (46. Industry standard, 96-well plates. QuickSync. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). Instrument settings can beIntegrating ValitaTiter and Molecular Devices fluorescence polarization (FP) plate readers produces a seamless IgG titer assay setup and optimization. SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701). Selects either 96-well or 384-well plate for a stand-alone read. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Primary considerations when choosing a microplate reader are throughput and flexibility. Spectrofluorometer from Molecular Devices® provides a flexible environment. Or scan up to 384 wells at any time to determine the best assay conditions. 4 (Molecular Devices cat. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. Cell viability and cytoxicity assays. Well-to-WellPrecision RFULinearity RelativeFluorescenceUnit(RFU)ScaleRatio(GeminiEM,XPS,SpectraMaxM2-M5e, FlexStation3only). VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. Please click on the links below to view the Microplate Reader Comparison Tables. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Deep well blocks available in 24, 96 and 384-well formats An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. Ensure ongoing compliance of your Molecular Devices microplate readers and be audit ready with comprehensive . This robust system is capable of reading 96 wells, each with its own sample beam and reference detector. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro densitometry of TLC plates and electrophoresis gels. This reader can also be used as a standard. 314 low lamp level warning. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. FAST AND ACCURATE METERTECH Microplate Reader $3,650. for molecular biology. 005 OD Normal and Rapid modes, 96- and 384-well plates;. 00. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. The rate of molecular motion of Protein G slows down when it is bound to antibodies, resulting in an increase of the FP value (Figure 2). Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Quartz 96 Well Plates | 96 Well Plate Flat Bottom | 96 Well Plate Reader | 96 Well Glass. The opaque walls prevent stray light from being detected from neighbouring. It offers extremely low absorbance background, excellent. 303 unable to cal dark current. Click SoftMax Pro Software User Guide. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. Corning. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Up to 96 well plates, there is often a good signal that can easily be detected, and 96 wells are measured comparatively quickly. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. com Molecular Devices (Germany) GmbH ismarcring ierach an der iss erman ©2019 Molecular Devices, LLC. B. Supersedes 1. 5 ng per well. wells across each plate • Enables viewing and tracking of every well in every plate • Reveals additional information on cellular. SoftMax Pro Software minimizes setup time for detection and automates standard curve fitting and sample quantitation. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. Compact designs make these instruments. Part of a plate can be selected so that only a selected area of the plate. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. 1 mL/well of media. Step 2: Add sample to well – antigen within the sample binds to the capture antibody. EMax. Category 2 and 3 microplate readers use gradient heating as well. . Supersedes 1. 2. BioTek Instruments Synergy Neo2 Multi-Mode Reader Fluorescence Measuring Equipment. In addition, the iD5 reader measures TRF and FP and. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Align the two slots on the bottle holder with the two pegs on the rail. * Note: Programming the washer with a final aspirate height of 4. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. Sort By: Show:. Performing molecular and cellular analyses in multiwell microplate formats is an important methodology for high-throughput screening, or for boosting throughput generally. Explore the best practices for running absorbance-based assays how Molecular Devices absorbance readers can be utilized to optimize said absorbance-based assays. Dynamic range > 6 decades . It is designed to work seamlessly with a variety of 96 well microplate readers, including popular brands such as Agilent™, Berthold™, Biotek™, BMG LABTECH™, Molecular Devices™, Perkin Elmer™, Promega™, Tecan™, and. Page 1 of 8 . 90349). 1 – 2. Wide range of applications The VersaMax reader covers a wide range of applications: Step 1: Turn on your microplate reader. Microplate Capacity. Plus 384. One plate received 200 µL of the BCA working reagent, and the other received 200 µL of the Rapid Gold BCA working reagent. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. The system can perform readings. Molecular Probes 96-well microplates for fluorescence-based assays are black-walled, clear-bottom 96-well microplates that minimize well-to-well crosstalk and. S. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. Mix samples in microplate wells by user-programmable Automix feature. Published Dec 16, 2020 | Updated Nov 07, 2022. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Fireflysci UV/VIS Fluorescence Validation Plate for Well Plate Reader (290-562nm) FLVP-01. High sensitivity and speed are required for these plate formats if you wish to examine quick kinetic measurements such as calcium fluxes or applications with small signal. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). Cell line development teams will realize savings on time and resources, as well as faster time-to-market by utilizing a single technology for measuring a critical quality attribute duringOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. 340 PC384. 5A spare fuses, 2 cleaning needles, tubing sets for all bottles, cleaning tool for needles. A control well was set up to verify. Optimized for fluorescence microplate readers. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. Site. Description Bottom Volume Qty/Pk Qty/Cs 3635 96 Well UV-Plate Flat 370 µL 2550per well of this semi-solid mixture was pipetted into 6-well plates. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. The BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. and glow luminescence modes in 96- to 384-well plates. 1 OD at 230 nm. 500 USD. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. 313 reference gain check fail. 96-, 384-, and 1536-well microplates. Description. In addition to increased throughput, microplate formats enable researchers to miniaturize their analysis (using less sample and reagent) to study regulatory events and. #ID5-STD) Pierce BCA Protein Assay Kit (ThermoFisher Scientific cat. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. Answer. The Best Microplate Readers According to our Product Reviewers (1) Write a Review. to each well. During preincubation, the working reagent was prepared by mixing fluorogenic substrate, assay buffer, and rFC enzyme solution in a 5:4:1 ratio, respectively. No accessories are needed and SoftMax® Pro Software. 96- and 384-well microplates. x. 5 OD at 492 nm Computer interface USB 2. 5 pM Luminescence performance Wavelength range 300–850 nm Wavelength selection Choice of simultaneous detection of all wavelengths or selection in 1. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. A = Log 10 (I 0 /I). Products : SpectraMax L reader How long does it take to dispense into a 96 well plate with SpectraMax L reader injection? For a 96-well plate it takes a total of 27. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Wells of 96-well plates (NEST) were pre-coated with 100 μg/ml of collagen solution for 1 hour. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 0 Optical system Filters Power requirementAfter reading the ELISA plate on the EMax® Plus Microplate Reader, the R2 value was 0. Product Compare 0. Evaporation protection (humidity cassette) Te-Cool™ (active temperature regulation 18-42 °C) Integrated lid handling. High tolerance for contaminants. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Multimodal plate reader: Yes: 25 mm: Yes—Max. 100 μL of each endotoxin standard was added to triplicate wells of an LAL Reagent Grade Multi-well Plate. *For Corning UV plates, mean values at 260 nm ranged from 0. Max, FlexStation, Gemini, and earlier instruments as well as import and analysis of data files from FLIPR and Analyst instruments. 9. They can be read by most microplate readers. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. External Dimensions without lid (mm). Molecular Spectroscopy Consumables & Accessories Thermal Analysis. Packed 25 plates per sleeve, 2 sleeves per case. No additional manuals are available. 190. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. as well as all other Molecular Devices' microplate readers. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. VIS absorbance detection. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. 00. The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. Never order or replace filters to optimize an assay again!6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. CO 2 & O 2 control. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. Validation made easy -1) Select the correct plate and the wells that you want to measure. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. x. 2b,c. • 0. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. 1 x plates; Instrument dimensions. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384-well microplates. Plate. 001 ABS (displayed), 0. Robust shaking and consistent temperature control optimize bacteria and yeast cell growth. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. Plate Readers, Fluorometers & Luminometers; Lab Automation; Molecular Diagnostics. 000 ABS • Resolution: 0. To validate the assays’ ability to detect a wide range of viable cell numbers, a series of cell densities was set up. The plate reader can measure multiple readouts, including UV and. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. 4: Berthold Technologies: TriStar LB 941: Multimodal plate reader: Yes:. This SpectraMax Plus comes from a long history of reliable tools built by Molecular Devices. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. 315 can't find zero order. . 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Self-righting base, up to 40 degrees past center without dispenser. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ Microplate Washer. The ImageXpress® Pico Automated Cell Imaging System is more than a digital microscope, combining high-resolution imaging with powerful analysis. VMax . Microplate Readers include the M3, M4, M5, and M5e models. Both the P and M injectors were set to deliver 100 µL (96-well plate) or 25 µL (384-well plate), with a 2-second delay and 10-second integration time. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life. VMax . Absorbance equation. Software Validation. The plate was preincubated in the reader at 37°C for a minimum of 10 minutes. Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. Access this by going to. 5. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. BMG Labtech - THERMOstar. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. Dual injectors for kinetic assays. The VersaMax reads 96-well microplates. 0 . Step 1: Capture antibody binds to ELISA plate wells. We would like to show you a description here but the site won’t allow us. Incubation. 2. 6-384 (96- and 384-well with pipetting) 96-well microplates. #3693; n = 4 for each standard). Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. In addition, users can define individual reagents and concentrations to be delivered to each well. Optional 12-way manifold) 5032336. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. (one set per assay) to clear 96-well microplates. Packed 25 plates per…. Washers configured for both 96- and 384-well plates. 984. #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. A 1:10 dilution series of ATP was set up in solid white 96- and 384-well plates at concentrations from 10 uM down to 1 nM, along with blank wells containing no cells. 76358-624. The picked. What is the dead volume in 96 and 384 well compound plates? Dead volume is 5 micro liters. #CA961) Methods. x. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm) with 4 filter choices included at time of purchase. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. 057, depending on lot number. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96-. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. The UV-Plate is compatible with the following plate readers: Bio-Tek PowerWave™ 200 Bio-Tek µQuant™ BioRad UltraMark™ Molecular Devices SPECTRAmax® 190, 250, 340PC, and Plus Ordering Information Cat. 00 USD. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. 4 (Molecular Devices cat. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13The microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. Each well of a 96- well microtiter plate is coated with a fluorescently labeled IgG-binding peptide, Protein G. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Other. Values are averages from 5 clean plates, each filled with 200 µL water/well. Microplate Reader. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. 100 μL of working reagent was added to each well after the 10-minute preincubation step. Two high-energy xenon flash lamps: Reading capabilities. (96- or 384-well) simultaneously and with high temporal resolution. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Learn more. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). Specifications subject to change without notice. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. EMax. U-bottom: 30-40 µl. Plate Storage Solutions for Liquid Handling Automation Remote Lab Management. 1. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Select up to 3 products. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Features: Standard back plate for use with hand sanitizer, hand lotion, or foam dispensers. \r Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. M2. 0. Bay Gray powder coated metal back plate, column, and base. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. 7°C (gradient heating design). x. Temperature control: 4°C above ambient to 45°C. The CellTiter-Glo assay was used to measure ATP. Z. 3. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Select models. Clear plates will give more cross talk between wells. Artel MVS Bundle DM Std/800TSNB Reader Details. These robust readers have been placed in labs from. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. 40266. This is equivalent to having 511 built-in filters, The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. Thermomax model provides thermal control and vertical shake, perfect for. Quartz Plate found in: Thick Quartz Ground and Polished Plates, Solid Quartz 96 Well Clear Plate with Clear Base, Quartz Parallel Plate Probes for DMA 7. Endpoint, kinetic, spectrum. Some instruments can handle only 96-well plates and lower, whereas others can read up to the ultrahigh throughput densities of 1,536-well plates. a white 96-well half-area microplate (Corning cat. and the A1 corner of the plate adapter (Figure 1-2). The VICTOR Nivo. Washer maintenance kit: 2 X T 2. MultiWash+ for 96-well plates: 4 liquid lines, 8-way manifold and tubing, 4 wash/rinse bottles (2L), 1 waste bottle (2. Versa Max. 300 µL of Detergent Compatible Bradford reagent was added to each well. Plus 384. Instrument settings can beCorning 96-well black microplates with clear bottom (Corning cat. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. 96-well plates - Filter plates with 0. Microplate optimization and read height optimization were performed for best results. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. Microplate optimization and read height optimization were performed for best results. They are also sealable using different types of adhesive mats foils or films. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. Mode Plate Reader. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the. TNF standard curve. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. Quantitative, whole well cell confluence for every well. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. MCF7 cells treated with. Multimode microplate reader equipped with latest detections like fluorescence,. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 2% in. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. All trademarks used herein are the property of. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Ensure that cells reach around 80–90% of confluence on the day of the. SpectraMax 250 User. Figure 3. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. It also integrates with the powerful SoftMax Pro Software for. Top reading gives greater sensitivity for 96 well plates, unless you have cells adhering to the bottom of the well. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Lucid Scientific. 000 – 4. Compare this item. The ELx808 TM Reader seamlessly interfaces with Lonza's WinKQCL TM Endotoxin Detection and Analysis Software. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. #SpectraMax L Config) Methods. Visit the supplier page or submit a quote to learn more. x. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. The screen on the left shows the wells selected for Method MI AF488-10 and Method MI AF488-5 for image acquisition from rows A-D of a 96-well plate at. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads the VMax Microplate Reader has both speed and performance. Absorbance values were read on the SpectraMax iD5 reader using a preconfigured BCA assay protocol (the SpectraMax iD3 reader is also suitable for these assays, as it has the same absorbance performance as the SpectraMax iD5 reader). The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. To test the optical clarity of the various microplates at low wavelengths, one well of each plate was filled with 100 μL (96-well) or 20 μL (384-well) distilled water, and its. A 1:10 dilution series of ATP was set up in solid white 96- and 384-well plates at concentrations from 10 uM down to 1 nM, along with blank wells containing no cells. 993 (Figure 3), validating the assay’s usefulness over a wide range of viable cell numbers. Published Dec 16, 2020 | Updated Dec 16, 2020. Molecular Devices SpectraMax Plus Microplate Reader Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader Sale Price: $6,495. #R8195. You should ensure that there is enough volume in compound plate wells to operate the IonWorks® system taking into account the dead volume. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite volume differences that may. Some will only support one well density (e. Two different agonists, acetylcholine and carbachol, were tested. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. Kinetic assays were read every 20-28 seconds for one hour,The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro Software. 100 ˜L (96-well) or 25 ˜L (384-well) of Loading Buffer was added to each assay well. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis.